PRICKLE2 polyclonal antibody

• Background :

  This gene encodes a homolog of Drosophila prickle. The exact function of this gene is not known, however, studies in mice suggest that it may be involved in seizure prevention. Mutations in this gene are associated with progressive myoclonic epilepsy type 5.

• Structure Composition :

  0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• Product Name Alternative :

  PRIC2; PRIC2_HUMAN; prickle-like 2 (Drosophila) ; prickle-like 2; Prickle-like protein 2; PRICKLE2

• Swiss Prot :

  Q7Z3G6

• Reactivity :

  Mouse

• Immunogen :

  KLH conjugated synthetic peptide derived from human Prickle 2:401-500/844

• Conjugation :

  Unconjugated

• Applications :

  IHC ICC IF

• Dilution :

  IHC 1:100-500 ICC 1:100-500 IF 1:100-500

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification :

  Unmodification

• Molecular Weight :

  ~ 96 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  PRICKLE2 polyclonal antibody detects endogenous levels of PRICKLE2 protein.

• Applications Notes :

  Paraformaldehyde-fixed, paraffin embedded (Mouse brain) ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Prickle 2) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.

• Host or Source :

  Rabbit

• CAS Number :

  9007-83-4