ERRγ polyclonal antibody

• Background:

  ERRβ/γ Antibody (E-1) is a high quality monoclonal ERRβ/γ antibody (also designated ERRβ/γ antibody) suitable for the detection of the ERRβ/γ protein of mouse, rat and human origin. ERRβ/γ Antibody (E-1) is available as both the non-conjugated anti-ERRβ/γ antibody form, as well as multiple conjugated forms of anti-ERRβ/γ antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Estrogen and progesterone receptors are members of a family of transcription factors that are regulated by the binding of their cognate ligands. The interaction of hormone-bound estrogen receptors with estrogen responsive elements (EREs) alters transcription of ERE-containing genes. Estrogen receptor-related proteins (ERRα, β and γ) are orphan nuclear receptors. Like estrogen receptors, ERRs bind specifically to EREs to activate reporter genes. ERRβ, also known as steroid hormone receptor ERR2 or estrogen receptor-like 2, is expressed during mammary gland development and is critical in embryo development. The loss of ERRβ results in severely impaired chorion formation leading to placental failure and embryonic death. ERRβ also potently represses the transcriptional activity of Nrf2. ERRγ, also known as ERR3, is abundantly expressed in fetal heart. The loss of ERRγ results in lactatemia and death within the first week of life.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

• Product Name Alternative:

  Estrogen-related receptor gamma; ERR gamma-2; Estrogen receptor-related protein 3; Nuclear receptor subfamily 3 group B member 3; ESRRG; ERR3; ERRG2; KIAA0832; NR3B3

• Swiss Prot:

  P62508

• Reactivity:

  Human

• Immunogen:

  Synthetic peptide, corresponding to Human ERRγ.

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB: 1:1000~1:2000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 60 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  ERRγ polyclonal antibody detects endogenous levels of ERRγ protein.

• Applications Notes:

  Western blot (WB) analysis of ERRγ polyclonal antibody at 1:1000 dilution Lane1:A549 whole cell lysate (40ug) Lane2:SGC7901 whole cell lysate (40ug) Lane3:Pancl whole cell lysate (40ug) Lane4:HCT116 whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4