UPF2 polyclonal antibody

• Background :

  This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD) . When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located in the perinuclear area. It interacts with translation release factors and the proteins that are functional homologs of yeast Upf1p and Upf3p. Two splice variants have been found for this gene; both variants encode the same protein.

• Structure Composition :

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative :

  Regulator of nonsense transcripts 2; Nonsense mRNA reducing factor 2; Up-frameshift suppressor 2 homolog; hUpf2; UPF2; KIAA1408, RENT2

• Swiss Prot :

  Q9HAU5

• Reactivity :

  Human, Rat

• Immunogen :

  Recombinant fusion protein containing a sequence corresponding to amino acids 1153-1272 of human UPF2 (NP_542166.1) .

• Conjugation :

  Unconjugated

• Applications :

  WB IHC

• Dilution :

  WB: 1:500~1:2000 IHC: 1:50~1:200

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification :

  Unmodification

• Molecular Weight :

  ~ 180 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  UPF2 polyclonal antibody detects endogenous levels of UPF2 protein.

• Applications Notes :

  Western blot analysis of extracts of various cell lines, using UPF2 antibody at 1:1000 dilution.

• Host or Source :

  Rabbit

• CAS Number :

  9007-83-4