TPP2 polyclonal antibody

• Background:

  Tripeptidyl-peptidase 2 (TPP2) is a well-conserved subtilisin-like amino peptidase that is expressed predominantly in the cytoplasmic compartment . The amino-terminal region of TPP2 harbors a catalytic triad that is characteristic of serine proteases and allows for TPP2 cleavage of tripeptides from the free amino terminus of oligopeptide substrates . TPP2 is a large (>5MDa) homooligomeric protease in which proteolytic activity is regulated by subunit oligomerization . While TPP2 plays a general role in amino acid homeostasis, research studies demonstrate that TPP2 is involved in MHC class I antigen presentation and DNA-damage repair . TPP2 activity is required for the survival of Burkitt's lymphoma cells, suggesting a possible role for TPP2 in oncogenesis . Additional research studies show that TPP2 proteolytic activity is important for regulating lysosome abundance and glycolytic metabolism and that TPP2 deficiency leads to defects in adaptive immunity, innate immunity, and nervous system development .

• Structure Composition:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Product Name Alternative:

  Tripeptidyl-peptidase 2; TPP-2; Tripeptidyl aminopeptidase; Tripeptidyl-peptidase II; TPP-II

• Swiss Prot:

  P29144

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant full length protein of human TPP2

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC, IF/ICC

• Dilution:

  WB (1/500 - 1/2000), IHC (1/50 - 1/200), IF/ICC (1/50 - 1/200)

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 135 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Recognizes endogenous levels of TPP2 protein.

• Applications Notes:

  Western blot analysis of TPP2 expression in Jurkat (A), SW620 (B), mouse brain (C), mouse spleen (D) whole cell lysates.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4