Products for Research Use Only

AAK1 polyclonal antibody

CAT: 0384-BS67123-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS67123-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis (PubMed:17494869, PubMed:11877457, PubMed:11877461, PubMed:12952931, PubMed:14617351, PubMed:25653444) .Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1 (PubMed:17494869) .
CAS Number
9007-83-4
Structure Composition
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Name Alternative
KIAA1048; AP2-associated protein kinase 1; Adaptor-associated kinase 1
Swiss Prot
Q2M2I8
Reactivity
Human, Mouse, Rat
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human AAK1. The exact sequence is proprietary.
Conjugation
Unconjugated
Applications
WB, IHC, IF/ICC
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100)
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 140 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of AAK1 protein.
Applications Notes
Western blot analysis of AAK1 expression in Hela (A) whole cell lysates.
Host or Source
Rabbit