POFUT1 polyclonal antibody

• Background :

  Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X (4,5) -[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Plays a crucial role in NOTCH signaling. Initial fucosylation of NOTCH by POFUT1 generates a substrate for FRINGE/RFNG, an acetylglucosaminyltransferase that can then extend the fucosylation on the NOTCH EGF repeats. This extended fucosylation is required for optimal ligand binding and canonical NOTCH signaling induced by DLL1 or JAGGED1. Fucosylates AGRN and determines its ability to cluster acetylcholine receptors (AChRs) .

• Structure Composition :

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Product Name Alternative :

  FUT12; KIAA0180; GDP-fucose protein O-fucosyltransferase 1; Peptide-O-fucosyltransferase 1; O-FucT-1

• Swiss Prot :

  Q9H488

• Reactivity :

  Human

• Immunogen :

  KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human POFUT1. The exact sequence is proprietary.

• Conjugation :

  Unconjugated

• Applications :

  WB, IHC, IF/ICC

• Dilution :

  WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification :

  Unmodification

• Molecular Weight :

  ~ 45 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  Recognizes endogenous levels of POFUT1 protein.

• Applications Notes :

  Western blot analysis of POFUT1 expression in K562 (A), HCT116 (B), HeLa (C) whole cell lysates.

• Host or Source :

  Rabbit

• CAS Number :

  9007-83-4