ULK1 polyclonal antibody

• Background:

  Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Serine/threonine-protein kinase ULK1; Autophagy-related protein 1 homolog (ATG1; Hatg1; Unc-51-like kinase 1; ULK1; KIAA0722

• Swiss Prot:

  O75385

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding to Human ULK1.

• Conjugation:

  Unconjugated

• Related Pathways:

  PI3K AKT signaling, MTOR signaling

• Applications:

  WB IHC

• Dilution:

  WB:1:500-1:1,000 IHC:1:50-1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 150 kDa

• Storage Conditions:

  Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  ULK1 polyclonal antibody detects endogenous levels of ULK1 protein.

• Applications Notes:

  Western blot (WB) analysis of ULK1 polyclonal antibody at 1:500 dilution LaneA:A549 whole cell lysate (40ug) LaneB:HEK293T whole cell lysate (40ug) LaneC:AML-12 whole cell lysate (40ug) LaneD:C6 whole cell lysate (40ug) LaneE:Panc1 whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4