ULK1 (S317) polyclonal antibody

CAT: 0384-AP1005-01Size: 50 µLDry Ice: NoHazardous: No
CAT#:0384-AP1005-01Size:50 µL
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Background
VPS34 (vacuolar protein sorting-associated protein 34), also known as VPT29, VPL7, PEP15 or END12, is a 875 amino acid peripheral membrane protein that localizes to the golgi apparatus. Belonging to the PI3/PI4-kinase family, VPS34 is involved in endosome-to-Golgi retrograde transport as part of the VPS34 PI3-kinase complex II. The VPS34 PI3-kinase complex II is required for the endosome-to-Golgi retrieval of PEP1 and KEX2, and the recruitment of VPS5 and VPS7, two components of the retromer complex, to endosomal membranes. It has been suggested that VPS34 may also be involved in ethanol tolerance and cell wall integrity.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Phosphatidylinositol 3-kinase catalytic subunit type 3 (EC:2.7.1.137) ; PI3-kinase type 3; PI3K type 3; PtdIns-3-kinase type 3; Phosphatidylinositol 3-kinase p100 subunit; Phosphoinositide-3-kinase class 3; hVps34; PIK3C3
Swiss Prot
O75385
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues in Human VPS34.
Conjugation
Unconjugated
Related Pathways
PI3K AKT signaling, Autophagy Signaling, MTOR signaling
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 150 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
VPS34 polyclonal antibody detects endogenous levels of VPS34 protein.
Applications Notes
Western blot (WB) analysis of ULK1 polyclonal antibody at 1:1000 dilution Lane1:L02 whole cell lysate (20ug) Lane2:Panc1 whole cell lysate (20ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:HCC827 whole cell lysate (40ug) Lane5:The Brain tissue lysate of Rat (40ug) Lane6:The Brain tissue lysate of Mouse (40ug)
Host or Source
Rabbit

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