Osteocalcin polyclonal antibody

• Background:

  This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  BGLAP; BGP; OC ; OCN; osteocalcin

• Swiss Prot:

  P02818

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Osteocalcin (BGLAP) (NP_954642.1) .

• Conjugation:

  Unconjugated

• Applications:

  WB IF/ICC

• Dilution:

  WB: 1:500~1:2000 IF/ICC: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 12 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Osteocalcin polyclonal antibody detects endogenous levels of Osteocalcin protein.

• Applications Notes:

  Western blot analysis of extracts of U-87MG cells, using Osteocalcin antibody at 1:1000 dilution.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4