ACC alpha polyclonal antibody

• Background:

  Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA . It is the key enzyme in the biosynthesis and oxidation of fatty acids . In rodents, the 265 kDa ACC1 (ACCα) form is primarily expressed in lipogenic tissues, while 280 kDa ACC2 (ACCβ) is the main isoform in oxidative tissues . However, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues . Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC . ACC is a potential target of anti-obesity drugs.

• Structure Composition:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Product Name Alternative:

  ACAC; ACC1; ACCA; Acetyl-CoA carboxylase 1; ACC1; ACC-alpha

• Swiss Prot:

  Q13085

• Reactivity:

  Human, Mouse, Rat, Bovine, Chicken, Sheep

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ACC alpha. The exact sequence is proprietary.

• Conjugation:

  Unconjugated

• Related Pathways:

  AMPK Signaling Pathway, Warburg Effect.

• Applications:

  WB, IHC

• Dilution:

  WB (1/500 - 1/1000), IHC (1/50 - 1/200)

• Purity:

  The antibody was purified by immunogen affinity chromatography.

• Modification:

  Unmodification

• Molecular Weight:

  ~ 280 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Recognizes endogenous levels of ACC alpha protein.

• Applications Notes:

  Western blot analysis of ACC alpha expression in PC12 (A), HEK293T (B), HCT116 (C) whole cell lysates.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4