Alpha-tubulin polyclonal antibody

• Background:

  The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins.

• Structure Composition:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Product Name Alternative:

  TUBA3; Tubulin alpha-1A chain; Alpha-tubulin 3; Tubulin B-alpha-1; Tubulin alpha-3 chain

• Swiss Prot:

  Q71U36; P68363

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Alpha-tubulin. The exact sequence is proprietary.

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB (1/500 - 1/1000)

• Purity:

  The antibody was purified by immunogen affinity chromatography.

• Modification:

  Unmodification

• Molecular Weight:

  ~ 55 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Recognizes endogenous levels of Alpha-tubulin protein.

• Applications Notes:

  Western blot analysis of Alpha-tubulin expression in mouse brain (A), rat brain (B), A549 (C), Hela (D) whole cell lysates.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4