LRP5 polyclonal antibody

• Background :

  LRP5 and LRP6 are single-pass transmembrane proteins belonging to the low-density lipoprotein receptor (LDLR) -related protein family. Unlike other members of the LDLR family, LRP5 and LRP6 have four EGF and three LDLR repeats in the extracellular domain, and proline-rich motifs in the cytoplasmic domain (1) . They function as co-receptors for Wnt and are required for the canonical Wnt/β-catenin signaling pathway (2,3) . LRP5 and LRP6 are highly homologous and have redundant roles during development (4,5) . The activity of LRP5 and LRP6 can be inhibited by the binding of some members of the Dickkopf (DKK) family of proteins (6,7) . Upon stimulation with Wnt, LRP6 is phosphorylated at multiple sites including Thr1479, Ser1490, and Thr1493 by kinases such as GSK-3 and CK1 (8-10) . Phosphorylated LRP6 recruits axin to the membrane and presumably activates β-catenin signaling (8-10) .LRP5 is involved in the regulation of bone homeostasis. Mutations and polymorphisms in LPR5 are associated with bone diseases like osteoporosis-pseudoglioma syndrome and high-bone-mass disorders (11-13) . In addition, mutations in LRP5 are found in patients with hyperparathyroid tumor and breast cancer (14,15) .

• Structure Composition :

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative :

  LRP5; BMND1; EVR1; EVR4; HBM; LR3; LRP7; OPPG; OPS; OPTA1; VBCH2

• Swiss Prot :

  O75197

• Reactivity :

  Mouse, Rat

• Immunogen :

  A synthetic peptide corresponding to a sequence within amino acids 1400-1500 of human LRP5 (NP_002326.2) .

• Conjugation :

  Unconjugated

• Related Pathways :

  Wntβ catenin signaling, Regulation of Microtubule Dynamics

• Applications :

  WB IF/ICC

• Dilution :

  WB: 1:100~1:500 IF/ICC: 1:50~1:200

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification :

  Unmodification

• Molecular Weight :

  ~ 180-200 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  LRP5 polyclonal antibody detects endogenous levels of LRP5 protein.

• Applications Notes :

  Western blot analysis of extracts of various cell lines, using LRP5 antibody at 1:500 dilution.

• Host or Source :

  Rabbit

• CAS Number :

  9007-83-4