LRP2 polyclonal antibody

• Background:

  The protein encoded by this gene, low density lipoprotein-related protein 2 (LRP2) or megalin, is a multi-ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney. This glycoprotein has a large amino-terminal extracellular domain, a single transmembrane domain, and a short carboxy-terminal cytoplasmic tail. The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase. The LRP2 protein is critical for the reuptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling; extracellular ligands include parathyroid horomones and the morphogen sonic hedgehog while cytosolic ligands include MAP kinase scaffold proteins and JNK interacting proteins. Recycling of this membrane receptor is regulated by phosphorylation of its cytoplasmic domain. Mutations in this gene cause Donnai-Barrow syndrome (DBS) and facio-oculoacoustico-renal syndrome (FOAR) .

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Low-density lipoprotein receptor-related protein 2; LRP-2; Glycoprotein 330; gp330; Megalin

• Swiss Prot:

  P98164

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant protein of human LRP2.

• Conjugation:

  Unconjugated

• Applications:

  WB IHC

• Dilution:

  WB 1:500 - 1:2000 IHC 1:50 - 1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 522 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  LRP2 polyclonal antibody detects endogenous levels of LRP2 protein.

• Applications Notes:

  Western blot analysis of extracts of mouse lung cells, using LRP2 antibody.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4