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WT1 polyclonal antibody

CAT: 0384-BS80006-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS80006-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.
CAS Number
9007-83-4
Structure Composition
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
WT1; AWT1; EWS-WT1; GUD; NPHS4; WAGR; WIT-2; WT33
Swiss Prot
P19544
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein of human WT1 (NP_001185480.1) .
Conjugation
Unconjugated
Applications
WB, IHC, IF/ICC
Dilution
WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
49kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Unmodification
Host or Source
Rabbit

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