BUB3 polyclonal antibody

• Background:

  This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

• Structure Composition:

  1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  BUB3; BUB3L; hBUB3

• Swiss Prot:

  O43684

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant fusion protein of human BUB3 (NP_004716.1) .

• Conjugation:

  Unconjugated

• Applications:

  WB, IF/ICC

• Dilution:

  WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  40kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Unmodification

• Applications Notes:

  Western blot analysis of extracts of various cell lines, using BUB3 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 90s.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4