BUB1 polyclonal antibody

• Background:

  Human cells contain two related protein kinases, BUB1 and BUBR1, that appear to have evolved from a single ancestral BUB1 gene. Both kinases are concentrated near the surface of the kinetochore where they monitor kinetochore-microtubule interactions. BUB1 and BUBR1 bind to kinetochores and are postulated to be components of the mitotic checkpoint, which monitors kinetochore activities to determine if chromosomes have achieved alignment at the spindle equator. BUBR1 is essential for normal mitotic progression as it prevents cells from prematurely entering anaphase. BUB3 is a conserved component of the mitotic spindle assembly complex and is also involved with the essential spindle checkpoint pathway that operates during early embryogenesis.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Mitotic checkpoint serine/threonine-protein kinase BUB1; hBUB1; BUB1A; BUB1; BUB1L

• Swiss Prot:

  O43683

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant full length Human BUB1.

• Conjugation:

  Unconjugated

• Applications:

  WB IHC

• Dilution:

  WB 1:500 - 1:2000 IHC 1:50 - 1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 122 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  BUB1 polyclonal antibody detects endogenous levels of BUB1 protein.

• Applications Notes:

  Western blot analysis of BUB1 polyclonal antibody

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4