BUB1 polyclonal antibody

CAT: 0384-BS6538-01Size: 50 µLDry Ice: NoHazardous: No
CAT#:0384-BS6538-01Size:50 µL
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Human cells contain two related protein kinases, BUB1 and BUBR1, that appear to have evolved from a single ancestral BUB1 gene. Both kinases are concentrated near the surface of the kinetochore where they monitor kinetochore-microtubule interactions. BUB1 and BUBR1 bind to kinetochores and are postulated to be components of the mitotic checkpoint, which monitors kinetochore activities to determine if chromosomes have achieved alignment at the spindle equator. BUBR1 is essential for normal mitotic progression as it prevents cells from prematurely entering anaphase. BUB3 is a conserved component of the mitotic spindle assembly complex and is also involved with the essential spindle checkpoint pathway that operates during early embryogenesis.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Mitotic checkpoint serine/threonine-protein kinase BUB1; hBUB1; BUB1A; BUB1; BUB1L
Swiss Prot
O43683
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant full length Human BUB1.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB 1:500 - 1:2000 IHC 1:50 - 1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 122 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
BUB1 polyclonal antibody detects endogenous levels of BUB1 protein.
Applications Notes
Western blot analysis of BUB1 polyclonal antibody
Host or Source
Rabbit

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