BUB1 polyclonal antibody

• Background:

  BUB1 mitotic checkpoint serine/threonine kinase (BUB1) Homo sapiens This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

• Structure Composition:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Product Name Alternative:

  BUB1; BUB1L; Mitotic checkpoint serine/threonine-protein kinase BUB1; hBUB1; BUB1A

• Swiss Prot:

  O43683

• Reactivity:

  Human

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human BUB1. AA range:781-830

• Conjugation:

  Unconjugated

• Applications:

  WB, ELISA

• Dilution:

  Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Modification:

  Unmodification

• Molecular Weight:

  ~ 120 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  BUB1 Polyclonal Antibody detects endogenous levels of BUB1 protein.

• Applications Notes:

  Western blot analysis of the lysates from HUVECcells using BUB1 antibody.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4