RFWD3 polyclonal antibody

• Background :

  E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage (PubMed:21504906, PubMed:21558276, PubMed:26474068, PubMed:28575657, PubMed:28575658) . Plays a key role in RPA-mediated DNA damage signaling and repair (PubMed:21504906, PubMed:21558276, PubMed:26474068, PubMed:28575657, PubMed:28575658, PubMed:28691929) . Acts by mediating ubiquitination of the RPA complex (RPA1, RPA2 and RPA3 subunits) and RAD51 at stalled replication forks, leading to remove them from DNA damage sites and promote homologous recombination (PubMed:26474068, PubMed:28575657, PubMed:28575658) . Also mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint (PubMed:20173098) . May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome (PubMed:20173098) . In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53 (PubMed:20173098) .

• CAS Number :

  9007-83-4

• Structure Composition :

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

• Product Name Alternative :

  FANCW; RNF201

• Swiss Prot :

  Q6PCD5

• Reactivity :

  Human

• Immunogen :

  The antiserum was produced against synthesized peptide derived from human RFWD3. AA range:374-423

• Conjugation :

  Unconjugated

• Applications :

  WB, IHC-P, ELISA

• Dilution :

  WB: 1/500-1/1000 IHC: 1/50-1/100 ELISA: 1/10000

• Purity :

  Affinity Purified

• Modification :

  Unmodified

• Molecular Weight :

  Calculated MW: 85 kDa; Observed MW: 85 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  IgG

• Applications Notes :

  Western blot analysis of RFWD3 in 823 lysates using RFWD3 antibody.

• Host or Source :

  Rabbit