Phospho-PERK (Thr980) Polyclonal Antibody

• Background:

  The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Jan 2010].

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  P-PERK (Thr980) ; PERK (Phospho Thr980) ; PERK (Phospho T980) ; HRI; HsPEK; Pancreatic eIF2-alpha kinase; PEK; PRKR like endoplasmic reticulum kinase; WRS; DKFZp781H1925; EC 2.7.11.1; EIF2AK3; Eukaryotic translation initiation factor 2 alpha kinase 3; Heme regulated EIF2 alpha kinase.

• Swiss Prot:

  Q9NZJ5

• Reactivity:

  Human, Mouse

• Immunogen:

  KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T980 of human PERK protein.

• Conjugation:

  Unconjugated

• Applications:

  WB IHC

• Dilution:

  WB: 1:500~1:1000 IHC: 1:100~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Phosphorylated

• Molecular Weight:

  ~ 140 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Phospho-PERK (Thr980) Polyclonal Antibody detects endogenous levels of Phospho-PERK protein only when phosphorylated at Thr980

• Applications Notes:

  Western blot analysis of PERK (Phospho-T980) expression in A375 (A), MCF7 (B), mouse testis (C) whole cell lysates.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4