Klotho Polyclonal Antibody

• Background:

  May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity) . Essential factor for the specific interaction between FGF23 and FGFR1. The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  KL antibody KLOT_HUMAN; Klotho peptide

• Swiss Prot:

  Q9UEF7

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human KL (NP_004786.2) .

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB=1:500-2000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 116 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Klotho Polyclonal Antibody detects endogenous levels of Klotho protein.

• Applications Notes:

  Western blot analysis of extracts of various cell lines, using KL antibody at 1:3000 dilution.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4