CES2 polyclonal antibody

• Background:

  CES1 and CES2 are the two major liver carboxylesterases which belong to the type-B carboxylesterase/lipase family. Helping the body in the detoxification of a wide range of xenobiotics, CES1 and CES2 are involved in the hydrolyzing activation of therapeutic ester and amide pro-drugs, as well as in the detoxification of several narcotic compounds. The catalytic activity of CES1 and CES2 is influenced by both the esterification site and the structure/moiety of the amino acid. While CES1 shows high affinity for aromatic and aliphatic esters, CES2 shows high affinity for 3,6-diacetyl and 6-monoacetly esters, such as those found in morphine and morphine derivatives.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Cocaine esterase; Carboxylesterase 2; CE-2; hCE-2; Methylumbelliferyl-acetate deacetylase 2; CES-2; ICE

• Swiss Prot:

  O00748

• Reactivity:

  Human, Mouse

• Immunogen:

  Synthetic peptide, corresponding to amino acids 379-424 of Human CES2.

• Conjugation:

  Unconjugated

• Applications:

  WB IHC

• Dilution:

  WB: 1:500~1:1000 IHC: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 70 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  CES2 polyclonal antibody detects endogenous levels of CES2 protein.

• Applications Notes:

  Western blot (WB) analysis of CES2 (Q369) pAb at 1:500 dilution Lane1:HepG2 whole cell lysate (40ug) Lane2:HCT116 whole cell lysate (40ug) Lane3:AML-12 whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4