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ATP5I (K69) polyclonal antibody

CAT: 0384-BS3813-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS3813-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Mitochondrial ATP synthases (ATPases) transduce the energy contained in membrane electrochemical proton gradients into the energy required for synthesis of high-energy phosphate bonds. ATPases contain two linked complexes: F1, the hydrophilic catalytic core; and F0, the membrane-embedded protein channel. The two complexes are linked by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled, via a rotary mechanism of the central stalk subunits, with proton translocation across the membrane. ATP5I, also known as mitochondrial ATP synthase subunit E or ATP5K, is a 69 amino acid protein member of the ATPase E subunit family. Localized to the inner membrane of the mitochondria, ATP5I is a part of the F0 complex.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
ATP synthase subunit e, mitochondrial; ATPase subunit e; ATP5I; ATP5K
Swiss Prot
P56385
Reactivity
Human, Mouse
Immunogen
Synthetic peptide, corresponding to amino acids 50-100 of Human ATP5I.
Conjugation
Unconjugated
Applications
WB IHC IP
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IP 1:50 - 1:100
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 12 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
ATP5I (K69) polyclonal antibody detects endogenous levels of ATP5I protein.
Applications Notes
Western blot (WB) analysis of ATP5I (K69) pAb at 1:500 dilution Lane1:AML-12 whole cell lysate (40ug) Lane2:C6 whole cell lysate (40ug) Lane3:HepG2 whole cell lysate (40ug) Lane4:PC3 whole cell lysate (40ug) Lane5:HEK293T whole cell lysate (40ug)
Host or Source
Rabbit

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