UVRAG (K699) polyclonal antibody

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. It is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection and cancer. The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. These proteins are involved in the formation of cytoplasmic vacuoles called autophagosomes that are delivered to lysosomes for degradation.The class III type phosphoinositide 3-kinase (PI3KC3) /Vps34 regulates vacuolar trafficking as well as autophagy. Multiple proteins have been shown to be associated with Vsp34, including: p105/Vsp15, Beclin-1, UVRAG, Atg14, and Rubicon, which can determine Vsp34 function. UVRAG (UV radiation resistance-associated gene) is associated with the Beclin-1/PI3KC3 complex and promotes PI3KC3 enzymatic activity and autophagy, while suppressing proliferation. Beclin-1 binding to UVRAG promotes both autophagosome maturation and endocytic trafficking. UVRAG is also a potential tumor suppressor protein with frameshift mutations observed in colon and gastric carcinomas.

9007-83-4

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

UV radiation resistance-associated gene protein; p63; UVRAG

Q9P2Y5

Human, Rat, Mouse

Synthetic peptide, corresponding to Human UVRAG.

Unconjugated

Autophagy Signaling

WB

WB: 1:1000~1:2000

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Unmodification

~ 90 kDa

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.

UVRAG (K699) polyclonal antibody detects endogenous levels of UVRAG protein.

Western blot (WB) analysis of UVRAG (K699) polyclonal antibody at 1:2000 dilution Lane1:MCF-7 whole cell lysate (40ug) Lane2:Jurkat whole cell lysate (40ug) Lane3:BV2 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug)