Cot (P284) polyclonal antibody

CAT: 0384-BS1074-01Size: 50 µLDry Ice: NoHazardous: No
CAT#:0384-BS1074-01Size:50 µL
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Background
The role of mitogen-activated protein kinases (MAPKs) in cell signaling pathways is well established. The rat gene Tpl-2, for tumor progression locus 2, and the human and mouse homologues c-Cot, for cancer osaka thyroid oncogene, encode a proto-oncogene serine/threonine protein kinase that was shown to play a role in the functional activation of the MAP kinase pathway. Overexpression of Cot induces MAP kinase activation in COS-1 and NIH/3T3 cells. Cot-mediated activation of MAP kinase is inhibited by both Ras N17, a dominant negative mutant of c-H-Ras, and Raf-1s621A, a dominant negative mutant of Raf-1, suggesting that Cot functions upstream of Ras and Raf-1.Other studies have shown that a kinase-negative, dominant negative mutant of Cot partially blocks Ras or Raf-1-induced MAP kinase activation, arguing that Cot functions downstream of Ras and Raf-1.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Mitogen-activated protein kinase kinase kinase 8; Cancer Osaka thyroid oncogene; Proto-oncogene c-Cot; Serine/threonine-protein kinase cot; Tumor progression locus 2; TPL-2; MAP3K8; COT; ESTF
Swiss Prot
P41279
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 250-300 of Human Cot.
Conjugation
Unconjugated
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 65 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
COT (P284) polyclonal antibody detects endogenous levels of COT protein.
Applications Notes
Western blot (WB) analysis of Cot (P284) pAb at 1:500 dilution Lane1:L02 whole cell lysate (40ug) Lane2:SGC7901 whole cell lysate (40ug) Lane3:PC3 whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug) Lane5:AML12 whole cell lysate (40ug)
Host or Source
Rabbit

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