Cot (P284) polyclonal antibody

• Background:

  The role of mitogen-activated protein kinases (MAPKs) in cell signaling pathways is well established. The rat gene Tpl-2, for tumor progression locus 2, and the human and mouse homologues c-Cot, for cancer osaka thyroid oncogene, encode a proto-oncogene serine/threonine protein kinase that was shown to play a role in the functional activation of the MAP kinase pathway. Overexpression of Cot induces MAP kinase activation in COS-1 and NIH/3T3 cells. Cot-mediated activation of MAP kinase is inhibited by both Ras N17, a dominant negative mutant of c-H-Ras, and Raf-1s621A, a dominant negative mutant of Raf-1, suggesting that Cot functions upstream of Ras and Raf-1.Other studies have shown that a kinase-negative, dominant negative mutant of Cot partially blocks Ras or Raf-1-induced MAP kinase activation, arguing that Cot functions downstream of Ras and Raf-1.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Mitogen-activated protein kinase kinase kinase 8; Cancer Osaka thyroid oncogene; Proto-oncogene c-Cot; Serine/threonine-protein kinase cot; Tumor progression locus 2; TPL-2; MAP3K8; COT; ESTF

• Swiss Prot:

  P41279

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding to amino acids 250-300 of Human Cot.

• Conjugation:

  Unconjugated

• Applications:

  WB IHC IF

• Dilution:

  WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 65 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  COT (P284) polyclonal antibody detects endogenous levels of COT protein.

• Applications Notes:

  Western blot (WB) analysis of Cot (P284) pAb at 1:500 dilution Lane1:L02 whole cell lysate (40ug) Lane2:SGC7901 whole cell lysate (40ug) Lane3:PC3 whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug) Lane5:AML12 whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4