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MSK1 (Phospho-Ser360) polyclonal antibody

CAT: 0384-BS4521-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS4521-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
MSK-1 is a mitogen and stress activated protein kinase-1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK-1can be activated by ERK1/2 and SAPK2 /p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG-14 in response to mitogen and stress. Similar to RSK, MSK-1 contains two kinase domains (N-term and a C-term) . Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK-1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C-terminal kinase domain of MSK-1 which is essential for the catalytic activity of the N-terminal kinase domain.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Product Name Alternative
Ribosomal protein S6 kinase alpha-5; S6K-alpha-5; 90 kDa ribosomal protein S6 kinase 5; Nuclear mitogen- and stress-activated protein kinase 1; RSK-like protein kinase; RSKL; RPS6KA5; MSK1
Swiss Prot
O75582
Reactivity
Human, Rat, Mouse
Immunogen
Synthetic phosphopeptide derived from human MSK1 around the phosphorylation site of Serine 360.
Conjugation
Unconjugated
Applications
WB IF
Dilution
WB: 1:500~1:1000 IF: 1:100~1:500
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylated
Molecular Weight
~ 100 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
MSK1 (Phospho-Ser360) polyclonal antibody detects endogenous levels of MSK1 protein only when phosphorylated at Ser360.
Applications Notes
Western blot (WB) analysis of MSK1 (Phospho-Ser360) polyclonal antibody at 1:500 dilution Lane1:NIH-3T3 whole cell lysate (30ug) Lane2:NIH-3T3 treated with Anisomycin (25μg/mL,30 minutes) whole cell lysate (15ug) Lane3:HCT116 whole cell lysate (30ug) Lane4:PMVEC whole cell lysate (30ug)
Host or Source
Rabbit

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