MITF (G174) polyclonal antibody

• Background:

  Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR) . Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1) . Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

• Product Name Alternative:

  Microphthalmia-associated transcription factor; Class E basic helix-loop-helix protein 32; bHLHe32

• Swiss Prot:

  O75030

• Reactivity:

  Human, Rat, Mouse

• Immunogen:

  Synthetic peptide, corresponding to Human MITF.

• Conjugation:

  Unconjugated

• Related Pathways:

  Wntβ catenin signaling

• Applications:

  WB IF

• Dilution:

  WB: 1:500~1:2000 IF: 1:100~1:500

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 60-75 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  MITF polyclonal antibody detects endogenous levels of MITF protein.

• Applications Notes:

  Western blot (WB) analysis of MITF polyclonal antibody at 1:1000 dilution Lane1:The heart tissue lysate of Rat (30ug) Lane2:The kidney tissue lysate of Mouse (30ug) Lane3:CT-26 whole cell lysate (30ug) Lane4:PC12 whole cell lysate (30ug) Lane5:A2780 whole cell lysate (30ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4