Methacholine (bromide)

• Description:

  Methacholine (Acetyl-β-methylcholine) bromide is a potent muscarinic-3 (M3) agonist. Methacholine bromide acts directly on acetylcholine receptors on smooth muscle causing bronchoconstriction and airway narrowing. Methacholine bromide shows a high sensitivity to identify bronchial hyperresponsiveness (BHR) . Methacholine bromide can be used to measure airway hyperresponsiveness (AHR) as a diagnostic aid in the assessment of individuals with asthma-like symptoms and normal resting expiratory flow rates[1][2][3][4].

• Product Name Alternative:

  Acetyl-β-methylcholine (bromide)

• UNSPSC:

  12352211

• Hazard Statement:

  H315-H319-H335

• Target:

  MAChR

• Type:

  Reference compound

• Related Pathways:

  GPCR/G Protein; Neuronal Signaling

• Applications:

  Neuroscience-Neuromodulation

• Field of Research:

  Cardiovascular Disease

• Assay Protocol:

  https://www.medchemexpress.com/methacholine-bromide.html

• Solubility:

  10 mM in DMSO

• Smiles:

  CC (OC (C) =O) C[N+] (C) (C) C.[Br-]

• Molecular Formula:

  C8H18BrNO2

• Molecular Weight:

  240.14

• Precautions:

  P261-P264-P271-P280-P302+P352-P304+P340-P362+P364-P405-P501

• References & Citations:

  [1]Cohen J, et al. Relationship between airway responsiveness to neurokinin A and methacholine in asthma. Pulm Pharmacol Ther. 2005;18 (3) :171-176.|[2]Anderson SD, et al. Comparison of mannitol and methacholine to predict exercise-induced bronchoconstriction and a clinical diagnosis of asthma. Respir Res. 2009;10 (1) :4. Published 2009 Jan 23.|[3]Cockcroft DW. Methacholine challenge methods. Chest. 2008;134 (4) :678-680.|[4]Kabara S, et al. Differential effects of thiopental on methacholine- and serotonin-induced bronchoconstriction in dogs. Br J Anaesth. 2003 Sep;91 (3) :379-84.|[5]Folkerts G, et al. Bradykinin causes inhibition of methacholine-induced bronchoconstriction in vivo in mice. Naunyn Schmiedebergs Arch Pharmacol. 2001 Jul;364 (1) :53-8. |[6]Vitorasso RL, et al. Methacholine dose response curve and acceptability criteria of respiratory mechanics modeling. Exp Lung Res. 2020 Feb-Mar;46 (1-2) :23-31.

• Shipping Conditions:

  Room temperature

• Scientific Category:

  Reference compound1

• Clinical Information:

  Launched

• Isoform:

  MAChR3

• CAS Number:

  333-31-3