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Human Nuclear Antigen Antibody

CAT: 0013-GTR18081129-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18081129-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This human nuclear antigen antibody is part of a panel of reagents which recognizes subcellular organelles or compartments. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. Clone 235-1 antibody specifically detects an antigen associated with the human nuclei. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. The nuclear antigen antibody can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
Reactivity
Human
Immunogen
Nuclei of human myeloid leukemia biopsy cells were used as the immunogen for this Nuclear Antigen antibody.
Clonality
Monoclonal
Clone
235-1
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein G affinity chromatography
Dilution
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Nuclear Antigen antibody to be titered up or down for optimal performance.
Tested Applications
FACS, IF
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG1, kappa

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