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Nuclear Antigen Antibody

CAT: 0013-GTR18082384-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18082384-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This mAb recognizes an antigen associated with the nuclei in cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions.
Reactivity
Human, Rat
Immunogen
Human cell nuclei were used as the immunogen for the Nuclear Antigen antibody.
Clonality
Monoclonal
Clone
NUCM1-1
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein G affinity chromatography
Dilution
Immunofluorescence: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Titering of the Nuclear Antigen antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IF, IHC-P
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG1, kappa

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