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CDK1 Antibody / CDC2

CAT: 0013-GTR18080932-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18080932-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody recognizes a 34kDa protein, which is identified as cyclin dependent kinase 1 (Cdk1) or p34cdc2 protein kinase. CDK1 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. It is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated CDK1 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.
UniProt
P06493
Reactivity
Human
Immunogen
Purified recombinant human Cdk1 / p34cdc2 was used as the immunogen for this CDK1 antibody.
Clonality
Monoclonal
Clone
POH-1
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Protein G purified antibody
Dilution
Western blot: 0.5-2ug/ml, Immunohistochemistry (FFPE) : 2-4ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CDK1 antibody to be titered up or down for optimal performance.1. Staining of FFPE tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Tested Applications
IHC-P, WB
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG2a, kappa

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