Catenin Beta Antibody / CTNNB1

• Description:

  Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex.

• Specifications:

  Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P35222

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A recombinant partial protein sequence (within amino acids 600-781) from the human protein was used as the immunogen for the Catenin Beta antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2 κ

• Clone:

  CTNNB1/7759

• Applications:

  WB, IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the Catenin Beta antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Catenin Beta antibody is available for research use only.

• Storage Conditions:

  Aliquot the Catenin Beta antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the Catenin Beta antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, Cytoplasm

• Image Legend:

  Analysis of a HuProt (TM) microarray containing more than 19,000 full-length human proteins using Catenin Beta antibody (clone CTNNB1/7759) . Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.