GCLM Antibody / Glutamate-Cysteine Ligase Regulatory Subunit

• Description:

  g-glutamylcysteine synthetase (g-GCS) is the rate limiting enzyme for glutathione (L-g-glutamyl-L-cysteinylglycine, GSH) synthesis. GSH is ubiquitous in mammalian cells as a vital intra- and extracellular protective antioxidant. g-GCS is a heterodimer of a heavy catalytic subunit and a light regulatory subunit that is responsive to inflammation, phenolic antioxidants, heat shock, oxidants and cytokines. The human g-GCS gene encoding the 367 amino acid catalytic subunit maps to chromosome 6p12. The human g-GCS gene encoding the regulatory subunit maps to chromosome 1p22.1. The two subunits of g-GCS form a heterodimeric zinc metalloprotein that gains activity through formation of a reversible disulfide bond.

• UniProt:

  P48507

• Host:

  Mouse

• Immunogen:

  Recombinant full-length human GLCLR protein was used as the immunogen for the GCLM antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Applications:

  IHC-P

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the GLCLR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This GLCLR antibody is available for research use only.

• CAS Number:

  9007-83-4