GCLM Antibody / Glutamate-Cysteine Ligase Regulatory Subunit

GCLM Antibody / Glutamate-Cysteine Ligase Regulatory Subunit

• Description: g-glutamylcysteine synthetase (g-GCS) is the rate limiting enzyme for glutathione (L-g-glutamyl-L-cysteinylglycine, GSH) synthesis. GSH is ubiquitous in mammalian cells as a vital intra- and extracellular protective antioxidant. g-GCS is a heterodimer of a heavy catalytic subunit and a light regulatory subunit that is responsive to inflammation, phenolic antioxidants, heat shock, oxidants and cytokines. The human g-GCS gene encoding the 367 amino acid catalytic subunit maps to chromosome 6p12. The human g-GCS gene encoding the regulatory subunit maps to chromosome 1p22.1. The two subunits of g-GCS form a heterodimeric zinc metalloprotein that gains activity through formation of a reversible disulfide bond.
• UniProt: P48507
• Host: Mouse
• Immunogen: Recombinant full-length human GCLM protein was used as the immunogen for the GCLM antibody.
• Clonality: Monoclonal
• Isotype: IgG2a κ
• Applications: IHC-P
• Format: Purified
• Buffer: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Reconstitution: Aliquot the GCLM antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
• Limitations: This GCLM antibody is available for research use only.