CD31 Antibody

CAT: 0800-V4534SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
CAT#:0800-V4534SAF-100UGSize:100 µg
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Description
CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 mAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P16284
Host
Mouse
Reactivity
Human
Immunogen
A recombinant partial protein sequence (within amino acids 625-738) from the human protein was used as the immunogen for the CD31 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RPECAM1/8832
Type
Recombinant
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Aliquot the CD31 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This CD31 antibody is available for research use only.
Storage Conditions
Aliquot the CD31 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the CD31 antibody should be determined by the researcher.
Location
Cell surface, Cytoplasm
Image Legend
IHC staining of FFPE human placental tissue with CD31 antibody (clone rPECAM1/8832) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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