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BRCA2 Antibody

CAT: 0800-V4319-20UGSize: 20 µgDry Ice: NoHazardous: No
CAT#:0800-V4319-20UGSize:20 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
In 1990, a breast cancer susceptibility gene designated BRCA1 was localized to chromosome 17q. Mutations within this gene are believed to account for approximately 45% of families with high incidence of breast cancer and at least 80% of families with increased incidence of both early-onset breast cancer and ovarian cancer. A second breast cancer susceptibility gene, BRCA2 (breast cancer 2, early onset), located on chromosome 13q13.1, also confers a high incidence of breast cancer but, unlike BRCA1, does not confer a substantially elevated risk of ovarian cancer. Both BRCA1 and BRCA2 play a role in the maintainance of genome stability, particularly in the homologous recombination pathway for double-strand DNA repair. BRCA2 is regarded as a tumor suppressor gene; tumors with BRCA2 mutations exhibit loss of heterozygosity (LOH) of the wildtype allele. The protein encoded by the BRCA2 gene contains multiple copies of a 70 amino acid motif called the BRC motif. These motifs effect binding to the Rad51 recombinase, which operates in DNA repair.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
P51587
Host
Mouse
Reactivity
Human
Immunogen
A recombinant partial protein sequence (within amino acids 200-500) from the human protein was used as the immunogen for the BRCA2 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
BRCA2/2158
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the BRCA2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This BRCA2 antibody is available for research use only.
Storage Conditions
Aliquot the BRCA2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the BRCA2 antibody should be determined by the researcher.
Location
Nucleus, Cytoplasm
Image Legend
IHC staining of FFPE human salivary gland tissue with BRCA2 antibody (clone BRCA2/2158) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Product Datasheet
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