BCCIP Antibody / BRCA2 and CDKN1A-interacting protein

• Specifications:

  Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

• UniProt:

  Q9P287

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  E.coli-derived human BCCIP recombinant protein (Position: K167-K300) was used as the immunogen for the BCCIP antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IHC, ICC, IF, FACS, ELISA

• Purity:

  Immunogen affinity purified

• Format:

  Lyophilized

• Buffer:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• Limitations:

  This BCCIP antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the BCCIP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

• Applications Notes:

  Optimal dilution of the BCCIP antibody should be determined by the researcher.

• Location:

  Nuclear

• Image Legend:

  Immunohistochemical staining of BCCIP using anti-BCCIP antibody. BCCIP was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-BCCIP antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.