BRCA1 Antibody

• Description:

  BRCA1 (Breast cancer type 1 susceptibility protein) is a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC) . This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene.

• Specifications:

  Immunohistochemistry (FFPE) : 2-5 µg/mL for 60 min at RT

• UniProt:

  P38398

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Amino acids 445-620 were used as the immunogen for the BRCA1 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  BRCA1/1398

• Applications:

  IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the BRCA1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This BRCA1 antibody is available for research use only.

• Storage Conditions:

  Store the BRCA1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the BRCA1 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nuclear, cytoplasmic

• Image Legend:

  IHC testing of FFPE human breast carcinoma tissue with BRCA1 antibody (clone BRCA1/1398) . HIER: boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min.