Basic Cytokeratin Antibody

• Description :

  6.0) subfamilies. This antibody recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1) ; 64kDa (CK3) ; 59kDa (CK4) ; 58kDa (CK5) ; 56kDa (CK6) ; 52kDa (CK8) . Many studies have shown the usefulness of keratin markers in cancer research and tumor diagnosis. The basic family clone AE3 is commonly used in combination with clone AE1 as a pan cytokeratin antibody acidic + basic cocktail (Cat No V2330) .Cat No V2330

• Specifications :

  Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 1-2 µg/mL, Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (2)

• Host :

  Mouse

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Solubilized cytokeratin extract from human stratum corneum was used as the immunogen.

• Clonality :

  Monoclonal

• Isotype :

  IgG1 k

• Clone :

  AE3

• Applications :

  WB, FACS, IHC-P, IF

• Purity :

  Protein G purified

• Format :

  Purified

• Buffer :

  1X PBS, pH 7.4

• Reconstitution :

  Store the Basic Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This Basic Cytokeratin antibody AE3 is available for research use only. The formulation is not suitable for therapeutic or diagnostic testing.

• Storage Conditions :

  Store the Basic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number :

  9007-83-4

• Location :

  Cytoplasmic

• Image Legend :

  IHC: FFPE skin stained with Basic Cytokeratin antibody AE3.