IL-18 Antibody

Four structurally related IL-1 receptor ligands have been described. These include three agonists designated IL-1Alpha, IL-1beta and IL-1gamma/IL-18 and a specific receptor antagonist, IL-1RAlpha. IL-1Alpha and IL-1beta play critical roles in the regulation of the immune response and inflammation, serving as activators of T and B lymphocytes and NK (natural killer) cells. IL-18 (also referred to as IL-1gamma) has been shown to augment the secretion of IFN-gamma from T lymphocytes and increase NK cell activity in spleen cells. IL-18 exhibits 19% and 12% identity with IL-1Alpha and IL-1beta respectively over the 12 beta-strands of the beta-trefoil fold domain, which is a signature feature of the IL-1 family. The unusual leader sequence of IL-18 may be analogous to the IL-1beta pro-domain which must be cleaved by the serine protease ICE for optimal secretion and biological activity. Originally described as IGIF (IFN-gamma-inducing factor), IL-18 is induced by mouse liver subsequent to challenge with lipopolysaccharide (LPS) .

9007-83-4

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL

Q14116

Human

A recombinant partial protein (within amino acids 1-193) from the human protein was used as the immunogen for the IL-18 antibody.

IL18/7781

IHC-P, WB

Protein A/G affinity

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Aliquot the IL-18 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

This IL-18 antibody is available for research use only.

Aliquot the IL-18 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the IL-18 antibody should be determined by the researcher.

Secreted