RING2 Antibody / RNF2 / RING1B

CAT: 0800-RQ7199Size: 100 µgDry Ice: NoHazardous: No
CAT#:0800-RQ7199Size:100 µg
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Description
E3 ubiquitin-protein ligase RING2 is an enzyme that in humans is encoded by the RNF2 gene. Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2) . Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL
UniProt
Q99496
Host
Rabbit
Reactivity
Human
Immunogen
Recombinant human protein (amino acids M1-K336) was used as the immunogen for the RING2 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, Direct ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the RING2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This RING2 antibody is available for research use only.
Storage Conditions
After reconstitution, the RING2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the RING2 antibody should be determined by the researcher.
Location
Cytoplasmic, nuclear
Image Legend
IHC staining of FFPE human laryngeal squamous carcinoma tissue with RING2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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