RING1 Antibody / Polycomb complex protein RING1

• Specifications:

  Western Blot: 0.25-0.5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

• UniProt:

  Q06587

• Host:

  Rabbit

• Reactivity:

  Human, Rat

• Immunogen:

  E.coli-derived human RING1 recombinant protein (Position: N6-K387) was used as the immunogen for the RING1 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, ICC, IF, FACS, ELISA

• Purity:

  Immunogen affinity purified

• Format:

  Lyophilized

• Buffer:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• Limitations:

  This RING1 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the RING1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

• Applications Notes:

  Optimal dilution of the RING1 antibody should be determined by the researcher.

• Location:

  Nuclear

• Image Legend:

  Immunofluorescent staining of RING1 using anti-RING1 antibody (red) and anti-Beta Tubulin antibody (green) . RING1 was detected in immunocytochemical section of cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-RING1 antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. Cy3 Conjugated Goat Anti-Rabbit IgG and FITC Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used.