PE-Labeled Human HLA-A*03:01&B2M Tetramer Protein (Peptide free)

PE-Labeled Human HLA-A*03:01&B2M Tetramer Protein (Peptide free)

• Background: Major histocompatibility complex (MHC), group of genes that code for proteins found on the surfaces of cells that help the immune system recognize foreign substances. MHC proteins are found in all higher vertebrates. In human beings the complex is also called the human leukocyte antigen (HLA) system.
• Description: PE-Labeled Human HLA-A*03:01&B2M Tetramer Protein (HLM-HP2H6) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*03:01) & Ile 21 - Met 119 (B2M) (Accession # P04439-1 (HLA-A*03:01) & P61769 (B2M)).
• Specifications: PE-Labeled Human HLA-A*03:01&B2M Tetramer Protein is assembled by biotinylated monomer (HLM-H82Ey) and PE-labeled streptavidin. Biotinylated Human HLA-A*03:01&B2M Monomer Protein is produced by co-expression of HLA and B2M. This Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
• Expression Region: Gly 25 - Thr 305 (HLA-A*03:01) & Ile 21 - Met 119 (B2M)
• Host: HEK 293
• Target: HLA-A*0301 & B2M
• Conjugation: PE Labeled
• Tag: C-10×His
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Bioactivity: Immobilized PE-Labeled Human HLA-A*03:01&B2M Tetramer Protein (Cat. No. HLM-HP2H6) at 1 μg/mL (100 μL/well) can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.06-4 ng/mL (QC tested).
• Format: Powder
• Buffer: PBS, 1% BSA, pH7.4
• Additives: Trehalose
• Molecular Weight: 35.9 kDa and 11.7 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C, aviod light