PE-Labeled Human HLA-E*01:03&B2M&CMV UL40 (VMAPRTVLL) Tetramer Protein

PE-Labeled Human HLA-E*01:03&B2M&CMV UL40 (VMAPRTVLL) Tetramer Protein

• Background: HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.
• Description: PE-Labeled Human HLA-E*01:03&B2M&CMV UL40 (VMAPRTVLL) Tetramer Protein (HLU-HP2H5) is expressed from human 293 cells (HEK293). It contains AA Gly 22 - Ile 305 (HLA-E*01:03) & Ile 21 - Met 119 (B2M) & VMAPRTVLL peptide (Accession # P13747 (HLA-E*01:03) & P61769-1 (B2M) & VMAPRTVLL).
• Specifications: PE-Labeled Human HLA-E*01:03&B2M&CMV UL40 (VMAPRTVLL) Tetramer Protein is assembled by biotinylated monomer (HL0-H82E7) and PE-labeled streptavidin. Biotinylated Human HLA-E*01:03&B2M&CMV UL40 (VMAPRTVLL) Complex Protein is produced by co-expression of HLA and B2M loaded with CMV UL40 peptide. Biotinylated Human HLA-E*01:03&B2M&CMV UL40 (VMAPRTVLL) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
• Expression Region: Gly 22 - Ile 305 (HLA-E*01:03) & Ile 21 - Met 119 (B2M) & VMAPRTVLL peptide
• Host: HEK 293
• Target: HLA-E*0103 & B2M & CMV UL40 (VMAPRTVLL)
• Conjugation: PE Labeled
• Tag: C-10×His
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Bioactivity: Immobilized Human NKG2C&CD94, Fc Tag (Cat. No. NC4-H5258) at 5 μg/mL (100 μL/well) can bind PE-Labeled Human HLA-E*01:03&B2M&CMV UL40 (VMAPRTVLL) Tetramer Protein (Cat. No. HLU-HP2H5) with a linear range of 0.01-5 μg/mL (QC tested).
• Format: Powder
• Buffer: PBS, 1% BSA, pH7.4
• Additives: Trehalose
• Molecular Weight: 36.2 kDa and 13.7 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C, aviod light