Anti-Fibronectin Antibody

Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Dry Ice Shipment: No

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CAT:

14-42042-1

Size:

400 µg

Price:

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For price, please contact [email protected].

Anti-Fibronectin Antibody

Description: Mouse Monoclonal Antibody specific to Human Fibronectin
Alternative Name: FN, Cold-insoluble globulin, CIG [Cleaved into: Anastellin; Ugl-Y1; Ugl-Y2; Ugl-Y3]
Gene Name: FN1
NCBI Gene ID: FN1
UniProt: P02751
Cellular Locus: Secreted, extracellular space, extracellular matrix
Host: Mouse
Reactivity: Human
Immunogen: Human plasma fibronectin
Target Antigen: Fibronectin
Target: Fibronectin
Clonality: Monoclonal
Isotype: IgG1
Type: Antibody
Applications: WB, ELISA, Inhibition
Field of Research: Cell adhesion
Purification Method: Purified by immunoaffinity chromatography
Dilution: Reconstitute lyophilized antibody in 200ul of distilled water (final concentration 2.0mg/ml).
Format: Purified
Form: Lyophilized
Buffer: Phosphate Buffered Saline
Function: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed:3024962, PubMed:3900070, PubMed:3593230, PubMed:7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed:3024962, PubMed:3900070, PubMed:3593230, PubMed:7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed:34089617). {UniProtKB:P11276, PubMed:3024962, PubMed:34089617, PubMed:3593230, PubMed:3900070, PubMed:7989369}.; [Anastellin]: Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. {PubMed:11209058, PubMed:15665290, PubMed:19379667, PubMed:8114919}.
Additionnal Information: ELISA: 1-10ug/ml with immobilized antigen

Western Blot: 1-10ug/ml, reducing or non-reducing conditions

Cell Adhesion Inhibition Assay: 0.1-1.0ug/ml on 10ug/ml fibronectin-coated microwells inhibits adhesion of trypsinized NRK cells.

Immunohistochemistry: 1-10ug/ml, paraffin-embedded or frozen tissue sections with Proteinase K treatment for antigen retrieval.

These are recommended concentrations.

End users should determine optimal concentrations for their applications.
Storage Conditions: The stock solution (2. 0mg/ml) can be stored in aliquots at -20°C for 1 year or can be stored at 4°C for 6 months after adding 0.1% sodium azide. Prepare dilutions of stock solution immediately before use. Do not store diluted stock solution. Avoid repeated freeze-thaw cycles.
Specificity: This antibody recognizes an epitope in the cell-binding domain of human fibronectin and can inhibit cell adhesion.
Formulation: PBS, pH 7.4, 1% BSA, lyophilized.
Buffer pH: pH 7.4
Target Background: Fibronectin is a 440kDa glycoprotein of the extracellular matrix that binds to integrins, collagen, fibrin, and heparan sulfate proteoglycans. Fibronectin plays a major role in cell adhesion, growth, migration, and differentiation, and it is important for processes such as wound healing and embryonic development. Altered fibronectin expression, degradation, and organization has been associated with a number of pathologies, including cancer and fibrosis.

Complementary Product Range

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