Anti-Calreticulin Antibody

Anti-Calreticulin Antibody

• Description: Rabbit Polyclonal Antibody specific to Calreticulin Antibody
• Product Name Alternative: CRP55, Calregulin, Endoplasmic reticulum resident protein 60, ERp60, HACBP, grp60
• CAS Number: 9007-83-4
• Gene Name: CALR
• NCBI Gene ID: CALR
• UniProt: P27797
• Cellular Locus: Endoplasmic reticulum lumen, Cytoplasm, cytosol, Secreted, extracellular space, extracellular matrix, Cell surface, Sarcoplasmic reticulum lumen, Cytoplasmic vesicle, secretory vesicle, Cortical granule, Cytolytic granule
• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Bovine, Canine, Chicken, Guinea Pig, Monkey, Pig, Hamster, Rabbit, Sheep
• Immunogen: Human calreticulin synthetic peptide conjugated to KLH.
• Target Antigen: Calreticulin
• Target: Calreticulin
• Clonality: Polyclonal
• Type: Antibody
• Applications: WB
• Field of Research: Neuroscience
• Purification Method: Whole antiserum
• Concentration: Lot Specific
• Dilution: Dilute in PBS or medium which is identical to that used in the assay system.
• Format: Whole Antiserum
• Form: Liquid
• Buffer: Whole Antiserum
• Function: Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (PubMed:7876246). Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export (PubMed:11149926). Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity). Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy (By similarity). {UniProtKB:P28491, UniProtKB:Q8K3H7, PubMed:11149926, PubMed:7876246}.
• Additionnal Information: Immunoblotting: use at 1:5,000-1:10,000 dilution. A band of ~63 kDa is detected.
  
  These are recommended concentrations. User should determine optimal concentrations for their application.
  
  Positive control: HeLa cell lysate.
• Storage Conditions: This antibody is stable for at least one (1) year at -20°C.
• Specificity: This antibody recognizes human, mouse, rat, bovine, canine, chicken, guinea pig, monkey, pig, hamster, rabbit, and sheep calreticulin.
• Formulation: Whole antiserum
• Target Background: Calreticulin is a multifunctional highly conserved Ca+2-binding protein that is localized primarily in the endoplasmic reticulum (ER) but has also been detected in the nucleus and nuclear envelope. Like many other ER proteins, it has the conserved ER retention KDEL sequence (Lys-Asp-Glu-Leu) at its C-terminus. Calreticulin is made up of three domains: 1 180 residue N-terminal domain, a proline- rich P-domain (aa 189-288) that binds Ca+2 with high affinity and shares homology with calnexin and calmegin, and a 110 residue C-terminal domain that binds Ca+2 with low affinity but high capacity. Calreticulin appears to be involved in calcium storage and regulation, has molecular chaperone activity, and has been shown to be induced in response to various types of cell stress.