GRP78 (Bip) Antibody

• Background:

  GRP78 (also known as BiP) is a 78-kDa glucose-regulated protein and a master regulator of the unfolded protein response (UPR) . As a key ER chaperone, it ensures proper protein folding, assembly, and the clearance of misfolded proteins, especially under stress conditions. Its expression is controlled by an internal ribosome entry site (IRES), which becomes highly active during cellular stress, such as heat shock or oxidative damage. GRP78 is essential for maintaining neuronal homeostasis and preventing apoptosis, making it a critical player in neurodegenerative disease contexts. In neuroscience, GRP78 has demonstrated neuroprotective effects against glutamate toxicity and oxidative stress. Its levels are notably reduced in the brains of Alzheimer’s disease patients, highlighting its potential as a biomarker and therapeutic target. Additionally, GRP78 supports embryonic development and pluripotent cell survival, further underscoring its importance in neural cell viability. However, its overexpression under extreme stress may contribute to drug resistance in neuro-oncology, adding complexity to its therapeutic modulation.

• Description:

  Rabbit Anti-Human GRP78 (Bip) Polyclonal

• Specifications:

  Detects ~78kDa.

• Product Name Alternative:

  GRP78, GRP-78, Grp78, GRP 78, HSPA5, HSPA 5, Heat shock 70 kDa protein 5, Heat Shock 70kDa Protein 5, BiP, BIP, Binding immunoglobulin protein, Immunoglobulin heavy chain-binding protein, Immunoglobulin Heavy Chain Binding Protein, Endoplasmic reticulum lumenal Ca (2+) -binding protein grp78, Endoplasmic reticulum lumenal, Ca2+ binding protein grp78, Glucose Regulated Protein 78kDa, 78 kDa glucose-regulated protein, 78 kDa glucose regulated protein, HSCe70, MIF2, mBiP, Sez7, AL022860, AU019543, D2Wsu141e, D2Wsu17e, FLJ26106, GRP78_HUMAN

• UNSPSC:

  12352203

• UN Code:

  Non-hazardous

• Hazard Statement:

  Non-hazardous

• Gene ID:

  3309

• Swiss Prot:

  P11021

• Accession Number:

  NP_005338.1

• Cellular Locus:

  Endoplasmic Reticulum | Endoplasmic Reticulum Membrane | Melanosome

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse, Rat, Dog, Drosophila, Hamster

• Immunogen:

  Full length human GRP78 (Bip) his tagged at the N terminus

• Target:

  GRP78

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Polyclonal

• Applications:

  WB | IHC | ICC/IF | ELISA

• Validated Applications:

  WB, IHC, ICC/IF, ELISA

• Field of Research:

  Cancer | Heat Shock | Cell Signaling | Protein Trafficking | Chaperone Proteins | Tags and Cell Markers | Organelle Markers

• Purification:

  Protein A Purified

• Detection Range:

  0.5 µg/ml of SPC-180 was sufficient for detection of Grp78 in 10 µg of rat tissue lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

• Concentration:

  1 mg/ml

• Dilution:

  WB (1:2000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

• Weight:

  0.012

• Buffer:

  PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. Cho S., et al. (2007) Mol Cell Biol. 27 (1) : 368-83. 2. Yang Y., et al. (1998) J Biol Chem. 273: 25552-25555. 3. Luo S., et al (2006) 26 (15) : 5688-97. 4. Yu Z., et al. (1999) Exp Neurol. 15: 302-314. 5. Koomagi R., et al. (1999) Anticancer Res. 19: 4333-4336. 6. Laquerre S., et al. (1998) J. Virology. 72: 4940-4949. 7. Dong D., et al. (2005) Cancer Res. 65 (13) : 5785-91.

• Shipping Conditions:

  Blue Ice or 4ºC

• Storage Conditions:

  -20ºC

• Specificity:

  Detects ~78kDa.

• Species:

  Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster

• CAS Number:

  9007-83-4

• Location:

  Endoplasmic Reticulum | Endoplasmic Reticulum Membrane | Melanosome

• Immunogen Species:

  Human