VPS35 Antibody, Clone 5A9

CAT: 0400-SMC-603SSize: 12 µgDry Ice: NoHazardous: No
CAT#:0400-SMC-603SSize:12 µg
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Background
Vacuolar Protein Sorter 35 (VPS35) is a core component of the retromer complex, a critical intracellular trafficking system responsible for the endosome-to-Golgi retrieval of membrane proteins. This pathway is essential for maintaining protein homeostasis, synaptic function, and neuronal survival. Mutations in VPS35—most notably the D620N variant—have been directly linked to familial and sporadic forms of Parkinson’s disease (PD) . These mutations impair retromer function, leading to disrupted recycling of key neuronal receptors and transporters. Dysfunctional VPS35 also contributes to mitochondrial fragmentation, oxidative stress, and impaired autophagy—cellular processes that are central to the pathogenesis of neurodegenerative diseases. Beyond Parkinson’s, VPS35 dysfunction has been implicated in Alzheimer’s disease and other proteinopathies, where it affects the trafficking of amyloid precursor protein (APP) and tau. Its role in regulating endosomal sorting and mitochondrial dynamics positions VPS35 as a critical node in the cellular pathways that maintain neuronal integrity. As a result, VPS35 is emerging as a promising therapeutic target in neurodegenerative disease research. Restoring or enhancing retromer function may offer novel strategies to counteract protein misfolding, synaptic loss, and neuroinflammation.
Description
Mouse Anti-Human VPS35 Monoclonal IgG1
CAS Number
9007-83-4
Specifications
Detects ~92 kDa.
Product Name Alternative
VPS35, VPS35 Retromer Complex Component, Vacuolar Protein Sorting-Associated Protein 35, Vacuolar Protein Sorting 35 Homolog, Vesicle Protein Sorting 35, MEM3, PARK17, FLJ10752, HVPS35, Maternal-Embryonic 3, TCCCTA00141
Synonyms
VPS35 Antibody, Clone 5A9
UNSPSC
12352203
UN Code
Non-hazardous
Hazard Statement
Non-hazardous
Gene ID
55737
Swiss Prot
Q96QK1
Accession Number
NP_060676.2
Cellular Locus
Endosome | Lysosome | Vesicles | Cytoplasm | Membrane
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Immunogen
Full length recombinant human VSP35
Target
VPS35
Clonality
Monoclonal
Isotype
IgG1
Clone
5A9
Conjugation
Unconjugated
Tissue Specificity
Ubiquitous. Highly expressed in brain, colon, heart, kidney, ovary, placenta, skeletal muscle, small intestine, testis, thymus.
Type
Monoclonal
Applications
WB | ICC/IF | IP
Validated Applications
WB, ICC/IF, IP
Field of Research
Cell Signaling | Neuroscience |Neurodegeneration | Parkinson's Disease | Membrane Trafficking Proteins | Golgi Proteins | Protein Trafficking | Alzheimer's Disease
Purification
Protein G Purified
Limit Of Detection
A 1:1000 dilution of SMC-603 was sufficient for detection of VPS35 in 10 µg of SH-SY5Y by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Concentration
1 mg/ml
Dilution
WB (1:1000) ; ICC/IF (1:200) ; IP (1:200) ; optimal dilutions for assays should be determined by the user.; optimal dilutions for assays should be determined by the user.
Weight
0.012
Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Precautions
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
References & Citations
1. Vilarino-Guell, C. et al. (2011) Am J Hum Genet 89:162–167 2. Zimprich, A. et al. (2011) Am J Hum Genet 89:168–175 3. Rahman, A.A., Morrison, B.E. (2019) Neurosci 401:1-10.
Shipping Conditions
Blue Ice or 4ºC
Storage Conditions
-20ºC
Specificity
Detects ~92 kDa.
Background Reference 01
1. Vilarino-Guell, C. et al. (2011) Am J Hum Genet 89:162–167 2. Zimprich, A. et al. (2011) Am J Hum Genet 89:168–175 3. Rahman, A.A., Morrison, B.E. (2019) Neurosci 401:1-10.
Species
Human | Mouse | Rat
Location
Endosome | Lysosome | Vesicles | Cytoplasm | Membrane
Isotype
IgG1
Immunogen Species
Human

Related Products

CatalogName
SMC-604SVPS35 Antibody, Clone 8A3
SMC-602SVPS35 Antibody, Clone 7E4
SMC-605SVPS35 Antibody, Clone 10A8
SMC-605DVPS35 Antibody, Clone 10A8
SMC-606SVPS35 Antibody, Clone 11H10
SMC-602DVPS35 Antibody, Clone 7E4
SMC-604DVPS35 Antibody, Clone 8A3
SMC-606DVPS35 Antibody, Clone 11H10
SMC-602D-HRPVPS35 Antibody, Clone 7E4: HRP
SMC-606D-APCVPS35 Antibody, Clone 11H10: APC

Alternative Products

CatalogName
MBS8005685-01VPS35 Antibody, Clone 11H10
MBS8005661-01VPS35 Antibody, Clone 10A8
MBS8005637-01VPS35 Antibody, Clone 8A3
MBS8005613-01VPS35 Antibody, Clone 5A9
MBS8005589-01VPS35 Antibody, Clone 7E4
MBS8005707-01VPS35 Antibody, Clone 11H10: RPE
MBS8005704-01VPS35 Antibody, Clone 11H10: HRP
MBS8005682-01VPS35 Antibody, Clone 10A8: PerCP
MBS8005680-01VPS35 Antibody, Clone 10A8: HRP
MBS8005659-01VPS35 Antibody, Clone 8A3: RPE

Popular Products