VPS35 Antibody, Clone 8A3

CAT: 0400-SMC-604SSize: 12 µgDry Ice: NoHazardous: No
CAT#:0400-SMC-604SSize:12 µg
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Background
Vacuolar Protein Sorter 35 (VPS35) is a core component of the retromer complex, a critical intracellular trafficking system responsible for the endosome-to-Golgi retrieval of membrane proteins. This pathway is essential for maintaining protein homeostasis, synaptic function, and neuronal survival. Mutations in VPS35—most notably the D620N variant—have been directly linked to familial and sporadic forms of Parkinson’s disease (PD) . These mutations impair retromer function, leading to disrupted recycling of key neuronal receptors and transporters. Dysfunctional VPS35 also contributes to mitochondrial fragmentation, oxidative stress, and impaired autophagy—cellular processes that are central to the pathogenesis of neurodegenerative diseases. Beyond Parkinson’s, VPS35 dysfunction has been implicated in Alzheimer’s disease and other proteinopathies, where it affects the trafficking of amyloid precursor protein (APP) and tau. Its role in regulating endosomal sorting and mitochondrial dynamics positions VPS35 as a critical node in the cellular pathways that maintain neuronal integrity. As a result, VPS35 is emerging as a promising therapeutic target in neurodegenerative disease research. Restoring or enhancing retromer function may offer novel strategies to counteract protein misfolding, synaptic loss, and neuroinflammation.
Description
Mouse Anti-Human VPS35 Monoclonal IgG1
CAS Number
9007-83-4
Specifications
Detects ~92 kDa.
Product Name Alternative
VPS35, VPS35 Retromer Complex Component, Vacuolar Protein Sorting-Associated Protein 35, Vacuolar Protein Sorting 35 Homolog, Vesicle Protein Sorting 35, MEM3, PARK17, FLJ10752, HVPS35, Maternal-Embryonic 3, TCCCTA00141
Synonyms
VPS35 Antibody, Clone 8A3
UNSPSC
12352203
UN Code
Non-hazardous
Hazard Statement
Non-hazardous
Gene ID
55737
Swiss Prot
Q96QK1
Accession Number
NP_060676.2
Cellular Locus
Endosome | Lysosome | Vesicles | Cytoplasm | Membrane
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Immunogen
Full length recombinant human VSP35
Target
VPS35
Clonality
Monoclonal
Isotype
IgG1
Clone
8A3
Conjugation
Unconjugated
Tissue Specificity
Ubiquitous. Highly expressed in brain, colon, heart, kidney, ovary, placenta, skeletal muscle, small intestine, testis, thymus.
Type
Monoclonal
Applications
WB | ICC/IF | IP | IHC
Validated Applications
WB, ICC/IF, IP, IHC
Field of Research
Cell Signaling | Neuroscience |Neurodegeneration | Parkinson's Disease | Membrane Trafficking Proteins | Golgi Proteins | Protein Trafficking | Alzheimer's Disease
Purification
Protein G Purified
Limit Of Detection
A 1:1000 dilution of SMC-604 was sufficient for detection of VPS35 in 10 µg of SH-SY5Y by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Concentration
1 mg/ml
Dilution
WB (1:1000) ; ICC/IF (1:200) ; IP (1:200) ; IHC (1:100) ; optimal dilutions for assays should be determined by the user.; optimal dilutions for assays should be determined by the user.
Weight
0.012
Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Precautions
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
References & Citations
1. Vilarino-Guell, C. et al. (2011) Am J Hum Genet 89:162–167 2. Zimprich, A. et al. (2011) Am J Hum Genet 89:168–175 3. Rahman, A.A., Morrison, B.E. (2019) Neurosci 401:1-10.
Shipping Conditions
Blue Ice or 4ºC
Storage Conditions
-20ºC
Specificity
Detects ~92 kDa.
Background Reference 01
1. Vilarino-Guell, C. et al. (2011) Am J Hum Genet 89:162–167 2. Zimprich, A. et al. (2011) Am J Hum Genet 89:168–175 3. Rahman, A.A., Morrison, B.E. (2019) Neurosci 401:1-10.
Species
Human | Mouse | Rat
Location
Endosome | Lysosome | Vesicles | Cytoplasm | Membrane
Isotype
IgG1
Immunogen Species
Human

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