KDEL Antibody

• Background:

  The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). Grp78 and Grp94 and PDI all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER.

• Description:

  Mouse Anti-KDEL Monoclonal IgG1

• Specifications:

  Detects KDEL tagged proteins.

• Product Name Alternative:

  KDEL Antibody, Lys Asp Glu Leu Antibody, Lys-Asp-Glu-Leu (KDEL) Antibody, Lys-Asp-Glu-Leu Antibody, Lysine-aspartic acid-glutamate-leucine Antibody

• Synonyms:

  KDEL Antibody, Clone 2C1

• UNSPSC:

  12352203

• Host:

  Mouse

• Species Reactivity:

  Human, Mouse, Rat

• Immunogen:

  KDEL peptide (C-AVKDEL -COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA)

• Target:

  KDEL

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  2C1

• Conjugation:

  Unconjugated

• Validated Applications:

  WB, IHC

• Purification:

  Protein G Purified

• Limit Of Detection:

  A 1:1000 dilution of SMC-539 was sufficient for detection of KDEL in 10 µg of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

• Concentration:

  1 mg/ml

• Dilution:

  WB (1:1000); IHC (1:25); optimal dilutions for assays should be determined by the user.

• Weight:

  0.012

• Buffer:

  PBS pH7.4, 50% glycerol, 0.09% sodium azide

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. Ozawa K., et al. (2008) Mol Pharmacol. 74:1610. 2. Austin R.C., et al. (2003) J Biol Chem. 278: 17438.

• CAS Number:

  9007-83-4