KDEL Antibody

• Background :

  The endoplasmic reticulum (ER) plays a central role in protein folding, quality control, and trafficking within eukaryotic cells. A key mechanism for retaining essential chaperones and folding enzymes within the ER involves the C-terminal KDEL sequence (Lys-Asp-Glu-Leu), a highly conserved ER retention motif. Proteins such as GRP78, GRP94, and protein disulfide isomerase (PDI) —critical for maintaining proteostasis—contain the KDEL motif, which ensures their retrieval from the Golgi apparatus back to the ER. This retention system is vital for sustaining ER function under both physiological and stress conditions. In the context of neurodegenerative diseases, such as Alzheimer’s, Parkinson’s, and ALS, chronic ER stress and impaired protein homeostasis are hallmark features. The KDEL retrieval system is increasingly recognized as a key modulator of the unfolded protein response (UPR), a cellular defense mechanism activated by misfolded proteins. Dysregulation of KDEL-containing chaperones can exacerbate protein aggregation, neuronal dysfunction, and cell death. Moreover, experimental evidence suggests that enhancing KDEL-mediated retention can reduce aberrant protein secretion and improve ER stress resilience, offering potential therapeutic avenues. As such, the KDEL motif and its associated retrieval machinery are emerging as critical components in the study of neurodegeneration and cellular stress adaptation.

• Description :

  Rabbit Anti-KDEL Polyclonal

• Specifications :

  Detects KDEL proteins, GRP94, Grp78, PDI and calreticulin. It may also see ERp57 and ERp29.

• Product Name Alternative :

  KDEL, K-D-E-L, Lys-Asp-Glu-Leu, Lys-Asp-Glu-Leu (KDEL), Lysine-aspartic acid-glutamate-leucine

• UNSPSC :

  12352203

• UN Code :

  Non-hazardous

• Hazard Statement :

  Non-hazardous

• Cellular Locus :

  Endoplasmic Reticulum

• Host :

  Rabbit

• Species Reactivity :

  Human, Mouse, Rat

• Immunogen :

  KDEL containing peptide immunogen

• Target :

  KDEL

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Polyclonal

• Applications :

  WB | ICC/IF | IHC

• Validated Applications :

  WB, ICC/IF, IHC

• Field of Research :

  Cell Signaling | Tags and Cell Markers | Organelle Markers | Protein Trafficking | Chaperone Proteins

• Purification :

  Protein A Purified

• Detection Range :

  A 1:1000 dilution of SPC-109 was sufficient for detection of KDEL-containing proteins in 20 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-mouse IgG as the secondary.

• Concentration :

  1 mg/ml

• Dilution :

  WB (1:1000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

• Weight :

  0.012

• Buffer :

  PBS pH7.2, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

• Precautions :

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations :

  1. Ozawa K., et al. (2008) Mol Pharmacol. 74:1610. 2. Austin R.C., et al. (2003) J Biol Chem. 278: 17438.

• Shipping Conditions :

  Blue Ice or 4ºC

• Storage Conditions :

  -20ºC

• Specificity :

  Detects KDEL proteins, GRP94, Grp78, PDI and calreticulin. It may also see ERp57 and ERp29.

• Species :

  Human | Mouse | Rat

• CAS Number :

  9007-83-4

• Location :

  Endoplasmic Reticulum